Matches in SemOpenAlex for { <https://semopenalex.org/work/W2376445561> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2376445561 abstract "A cDNA subtractive library of Monascus was constructed by using suppression subtractive hybridization technique. The library only contains the differently expressing cDNAs between Monascus producing citrinin and not producing citrinin. mRNA was isolated from mycelium of Monascus. Single-strand cDNAs and double-strand cDNAs were synthesized in turn. After enzyme restriction, cDNAs between 250-750bp were obtained. cDNAs from Monascus that producing citrinin then were divided into two groups and ligated to the specific adaptor1 and adaptor 2 respectively. After the cDNAs hybridized twice with cDNAs from Monascus that not producing citrinin and underwent two times of nested PCR, then linked with arms of T/A plasmid vectors, the subtractive library was set up. Amplification of the library was carried out with the E.coli strain JM109. The amplified library contains 283 positive clones. Rapid analysis by PCR shows that all plasmids in the clones contain 250-750bp inserts. The constructed cDNA subtractive library of Monascus lays solid foundation for screening key genes from citrinin biosynthetic pathway in species of Monascus." @default.
- W2376445561 created "2016-06-24" @default.
- W2376445561 creator A5033485760 @default.
- W2376445561 date "2003-01-01" @default.
- W2376445561 modified "2023-09-25" @default.
- W2376445561 title "CONSTRUCTION OF A CDNA SUBTRACTIVE LIBRARY OF MONASCUS" @default.
- W2376445561 hasPublicationYear "2003" @default.
- W2376445561 type Work @default.
- W2376445561 sameAs 2376445561 @default.
- W2376445561 citedByCount "0" @default.
- W2376445561 crossrefType "journal-article" @default.
- W2376445561 hasAuthorship W2376445561A5033485760 @default.
- W2376445561 hasConcept C100544194 @default.
- W2376445561 hasConcept C104317684 @default.
- W2376445561 hasConcept C13575015 @default.
- W2376445561 hasConcept C153911025 @default.
- W2376445561 hasConcept C167625842 @default.
- W2376445561 hasConcept C187882448 @default.
- W2376445561 hasConcept C189482833 @default.
- W2376445561 hasConcept C196436469 @default.
- W2376445561 hasConcept C22744801 @default.
- W2376445561 hasConcept C2776534934 @default.
- W2376445561 hasConcept C2777064906 @default.
- W2376445561 hasConcept C31903555 @default.
- W2376445561 hasConcept C54355233 @default.
- W2376445561 hasConcept C59822182 @default.
- W2376445561 hasConcept C84699730 @default.
- W2376445561 hasConcept C86803240 @default.
- W2376445561 hasConceptScore W2376445561C100544194 @default.
- W2376445561 hasConceptScore W2376445561C104317684 @default.
- W2376445561 hasConceptScore W2376445561C13575015 @default.
- W2376445561 hasConceptScore W2376445561C153911025 @default.
- W2376445561 hasConceptScore W2376445561C167625842 @default.
- W2376445561 hasConceptScore W2376445561C187882448 @default.
- W2376445561 hasConceptScore W2376445561C189482833 @default.
- W2376445561 hasConceptScore W2376445561C196436469 @default.
- W2376445561 hasConceptScore W2376445561C22744801 @default.
- W2376445561 hasConceptScore W2376445561C2776534934 @default.
- W2376445561 hasConceptScore W2376445561C2777064906 @default.
- W2376445561 hasConceptScore W2376445561C31903555 @default.
- W2376445561 hasConceptScore W2376445561C54355233 @default.
- W2376445561 hasConceptScore W2376445561C59822182 @default.
- W2376445561 hasConceptScore W2376445561C84699730 @default.
- W2376445561 hasConceptScore W2376445561C86803240 @default.
- W2376445561 hasLocation W23764455611 @default.
- W2376445561 hasOpenAccess W2376445561 @default.
- W2376445561 hasPrimaryLocation W23764455611 @default.
- W2376445561 hasRelatedWork W1573306281 @default.
- W2376445561 hasRelatedWork W1970793250 @default.
- W2376445561 hasRelatedWork W1977221760 @default.
- W2376445561 hasRelatedWork W2004485736 @default.
- W2376445561 hasRelatedWork W2026219787 @default.
- W2376445561 hasRelatedWork W2039391583 @default.
- W2376445561 hasRelatedWork W2143840985 @default.
- W2376445561 hasRelatedWork W2264525843 @default.
- W2376445561 hasRelatedWork W2290474586 @default.
- W2376445561 hasRelatedWork W2387131845 @default.
- W2376445561 hasRelatedWork W2415219992 @default.
- W2376445561 hasRelatedWork W2417906330 @default.
- W2376445561 hasRelatedWork W2482368129 @default.
- W2376445561 hasRelatedWork W2590475899 @default.
- W2376445561 hasRelatedWork W3149388296 @default.
- W2376445561 hasRelatedWork W3187594035 @default.
- W2376445561 hasRelatedWork W1897183072 @default.
- W2376445561 hasRelatedWork W2160742793 @default.
- W2376445561 hasRelatedWork W2396288022 @default.
- W2376445561 hasRelatedWork W2848206407 @default.
- W2376445561 isParatext "false" @default.
- W2376445561 isRetracted "false" @default.
- W2376445561 magId "2376445561" @default.
- W2376445561 workType "article" @default.