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- W2376450003 abstract "Objective To construct replication defective adenovirus that was recombinant with Survivin promoter and LRIG1 gene,and lay the foundation for following cancer gene therapy experiments.MethodsThe Survivin promoter was linked to pLRIGl-GFP and pEGFP-Surp-LRIGl was constructed,and the pEGFP-Surp-LRIGl recombinant plasmid was transfected into 293 cell with Pbhge3 containing adenovirus type 5 that included Right Arm,which produced Ad-Surp-LRIGl by the homologous recombination method.Ad-Surp-LRIGl was propagated in 293 cells and purified by cesium chloride gradient centrifugation.ResultsIdentified by restriction endonuclease analysis and PCR,recombinant adenovirus with Survivin promoter and LRIG1 gene was constructed successfully,with a viral titer of 2.3×10pfu/ml.ConclusionsThe recombinant adenovirus carrying LRIG1 gene driven by Survivin promoter has been constructed successfully,which can be used to the research of gene therapy for bladder cancer." @default.
- W2376450003 created "2016-06-24" @default.
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- W2376450003 date "2012-01-01" @default.
- W2376450003 modified "2023-09-26" @default.
- W2376450003 title "Construction and identification of a recombinant adenoviral vector carrying LRIG1 gene driven by Survivin promoter" @default.
- W2376450003 hasPublicationYear "2012" @default.
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