Matches in SemOpenAlex for { <https://semopenalex.org/work/W2376460685> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2376460685 abstract "Objective:To investigate the protective effects of schizandra B(SchB)on the injury of human neuroblastoma SH-SY5 Y cells induced by glutamic acid(Glu),and to clarify its influence in ATR signaling pathway.Methods:The SH-SY5 Y cells were divided into control group,dimethyl sulfoxide(DMSO)group,model group,SchB1 group,SchB2 group,and SchB3 group.The SH-SY5 Y cells in control group were untreated;the SH-SY5 Ycells in DMSO group were treated with DMSO(17mmol·L-1)for 24h;the SH-SY5 Y cells in model group were treated with Glu(20mmol·L-1)for 24 h.The SH-SY5 Ycells in SchB1 group,SchB2 group,and SchB3 group were firstly pretreated with SchB(0.1μmol· L-1)for 24 h,and exposed to20mmol·L-1 Glu for 24 hat the same time.The survival rate of SH-SY5 Ycells was detected by MTT assay,and the changes of cell cycle of SH-SY5 Ycells were determined by flow cytometry.Western blotting method was used to detect the protein expressions.Results:The MTT detection results showed that compared with model group,the survival rates of SH-SY5 Ycells in SchB1 group,SchB2 group,and SchB3 group were increased(P0.05 or P0.01),and the survival rate in SchB2 group was the highest(P0.01),the SchB2 group was selected as SchB group for the following experiment.The cell cycle detection results showed that the percentage of SH-SY5 Ycells in model group at G0/G1 phage was decreased(P0.05);compared with control group,the percentages of SH-SY5 Ycells in model group at S phage and G2/M phage were increased(P0.05);compared with model group,the percentage of SH-SY5 Ycells in SchB group at G0/G1 phage was increased(P0.05),and the percentages of SH-SY5 Ycells in SchB group at S phage and G2/M phage were decreased(P0.05).The Western blotting detection results showed that compared with control group,the expression levels of ATR,pCHK1,p-Cdc25 cand P53proteins in the SH-SY5 Ycells in model group were increased(P0.05);compared with model group,the expression levels of ATR,p-CHK1,p-Cdc25 cand P53proteins in the SH-SY5 Ycell in SchB group were decreased(P0.05).Conclusion:SchB could protect the damage of SH-SY5 Ycells by GLu,and its mechanism may be related to inhibiting the ATR signaling pathway and reducing the cell cycle arrest." @default.
- W2376460685 created "2016-06-24" @default.
- W2376460685 creator A5065544156 @default.
- W2376460685 date "2016-01-01" @default.
- W2376460685 modified "2023-09-24" @default.
- W2376460685 title "Protective effect of schizandra B on injury of SH-SY5Y cells induced by Glu and its mechanim" @default.
- W2376460685 hasPublicationYear "2016" @default.
- W2376460685 type Work @default.
- W2376460685 sameAs 2376460685 @default.
- W2376460685 citedByCount "0" @default.
- W2376460685 crossrefType "journal-article" @default.
- W2376460685 hasAuthorship W2376460685A5065544156 @default.
- W2376460685 hasConcept C104317684 @default.
- W2376460685 hasConcept C126322002 @default.
- W2376460685 hasConcept C1491633281 @default.
- W2376460685 hasConcept C153911025 @default.
- W2376460685 hasConcept C178790620 @default.
- W2376460685 hasConcept C185592680 @default.
- W2376460685 hasConcept C2776715637 @default.
- W2376460685 hasConcept C2778452553 @default.
- W2376460685 hasConcept C2779083432 @default.
- W2376460685 hasConcept C2780678043 @default.
- W2376460685 hasConcept C2780783641 @default.
- W2376460685 hasConcept C29537977 @default.
- W2376460685 hasConcept C54355233 @default.
- W2376460685 hasConcept C553184892 @default.
- W2376460685 hasConcept C55493867 @default.
- W2376460685 hasConcept C71924100 @default.
- W2376460685 hasConcept C81885089 @default.
- W2376460685 hasConcept C85265743 @default.
- W2376460685 hasConcept C86803240 @default.
- W2376460685 hasConceptScore W2376460685C104317684 @default.
- W2376460685 hasConceptScore W2376460685C126322002 @default.
- W2376460685 hasConceptScore W2376460685C1491633281 @default.
- W2376460685 hasConceptScore W2376460685C153911025 @default.
- W2376460685 hasConceptScore W2376460685C178790620 @default.
- W2376460685 hasConceptScore W2376460685C185592680 @default.
- W2376460685 hasConceptScore W2376460685C2776715637 @default.
- W2376460685 hasConceptScore W2376460685C2778452553 @default.
- W2376460685 hasConceptScore W2376460685C2779083432 @default.
- W2376460685 hasConceptScore W2376460685C2780678043 @default.
- W2376460685 hasConceptScore W2376460685C2780783641 @default.
- W2376460685 hasConceptScore W2376460685C29537977 @default.
- W2376460685 hasConceptScore W2376460685C54355233 @default.
- W2376460685 hasConceptScore W2376460685C553184892 @default.
- W2376460685 hasConceptScore W2376460685C55493867 @default.
- W2376460685 hasConceptScore W2376460685C71924100 @default.
- W2376460685 hasConceptScore W2376460685C81885089 @default.
- W2376460685 hasConceptScore W2376460685C85265743 @default.
- W2376460685 hasConceptScore W2376460685C86803240 @default.
- W2376460685 hasLocation W23764606851 @default.
- W2376460685 hasOpenAccess W2376460685 @default.
- W2376460685 hasPrimaryLocation W23764606851 @default.
- W2376460685 hasRelatedWork W1540824853 @default.
- W2376460685 hasRelatedWork W2347459042 @default.
- W2376460685 hasRelatedWork W2353487233 @default.
- W2376460685 hasRelatedWork W2356785830 @default.
- W2376460685 hasRelatedWork W2363626654 @default.
- W2376460685 hasRelatedWork W2364203900 @default.
- W2376460685 hasRelatedWork W2364425913 @default.
- W2376460685 hasRelatedWork W2365724962 @default.
- W2376460685 hasRelatedWork W2379638453 @default.
- W2376460685 hasRelatedWork W2380967106 @default.
- W2376460685 hasRelatedWork W2388792625 @default.
- W2376460685 hasRelatedWork W2389927607 @default.
- W2376460685 hasRelatedWork W2391792022 @default.
- W2376460685 hasRelatedWork W2392333210 @default.
- W2376460685 hasRelatedWork W2393467374 @default.
- W2376460685 hasRelatedWork W2419282157 @default.
- W2376460685 hasRelatedWork W2728395459 @default.
- W2376460685 hasRelatedWork W28762122 @default.
- W2376460685 hasRelatedWork W3028619125 @default.
- W2376460685 hasRelatedWork W3032728687 @default.
- W2376460685 isParatext "false" @default.
- W2376460685 isRetracted "false" @default.
- W2376460685 magId "2376460685" @default.
- W2376460685 workType "article" @default.