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- W2376466728 abstract "Objective To construct expression recombinant plasmid containing the Hexon gene of Ad3 and express the protein to provide the foundation to produce genetic engineering vaccine.Methods Recombinant plasmid pUC19Hexon and vector pQE31 were purified and cut by restriction enzyme BamHⅠ and SalⅠ. The target fragment was then connected with vector pQE31 by ligase T4. Then expressing recombinant plasmid pQE31 Hexon was charactered by restriction enzyme digestion and sequencing. The fusion protein is expressed in E.coli M15. Results The expressing recombinant pQE31 Hexon was obtained.Meanwhile, it was demonstrated that the expressing fragment was Ad3 Hexon sequence indeed by aligning with Genebank. With the induction by IPTG, the target protein was expressed in E.coli.Conclusion The Ad3 Hexon protein is expressed in E.coli successfully." @default.
- W2376466728 created "2016-06-24" @default.
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- W2376466728 date "2004-01-01" @default.
- W2376466728 modified "2023-09-27" @default.
- W2376466728 title "Construction and expressing of Hexon gene of human adenovirus type 3" @default.
- W2376466728 hasPublicationYear "2004" @default.
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