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- W2376483089 abstract "To analyze gene sequence of the cattle Theileria sergenti in Jilin, a pair of primers was designed according to the T.S.P33 surface protein gene sequences published in the GenBank. The T.S. gene fragment was amplified by PCR and cloned to pGEM-T Easy vector. Positive clones were identified by PCR screening and EcoR ￠?restriction digestion. Sequencing of positive clones showed that the cloned gene has a total length of 868 bp, which encodes a protein of 283 amino acids with two potential glycosylation sites. Sequence comparison with Korean strain (AF521557), Japanese strain (AB016280), Russia strain ￡¨AB016279) showed that the cloned gene fragment has a homology of 99.4 %, 88.0 % and 88.1 % with respective strain." @default.
- W2376483089 created "2016-06-24" @default.
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- W2376483089 date "2007-01-01" @default.
- W2376483089 modified "2023-09-23" @default.
- W2376483089 title "Cloning and sequence analysis of the cattle Theileria sergenti P33 surface protein gene" @default.
- W2376483089 hasPublicationYear "2007" @default.
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