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- W2376519904 abstract "Objective To establish a simultaneous separation method for reference substances of kaempferol-3-O-β-D-glucuronide and kaempferol-3-O-β-D-glucoside from Cichorium glandulosum.Methods The EtOAc portion from the ethanol extract of C.glandulosum was subjected to column chromatography on silica gel.The collecting fractions was further purified by Sephdex LH-20 column chromatography and preparative HPLC.Their structures were identified by NMR and MS.The purities of the isolates was detected by TLC and HPLC-UV-MS.Results Two compounds were isolated and identified as kaempferol-3-O-β-D-glucuronide(1)and kaempferol-3-O-β-D-glucoside(2).The TLC and HPLC analysis showed that the purities of the these two compounds were both more than 98%.Compound 2 was isolated from this genus for the first time.Conclusion The two reference substances acquired by this method could meet the demands of the chemical reference substances in Chinese material medica1.It could be used as a reference standard of the quality control and the research of herbal medicine." @default.
- W2376519904 created "2016-06-24" @default.
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- W2376519904 date "2011-01-01" @default.
- W2376519904 modified "2023-09-23" @default.
- W2376519904 title "Preparation of kaempferol-3-O-β-D-glucuronide and kaempferol-3-O-β-D-glucoside reference substances from Cichorium glandulosum" @default.
- W2376519904 hasPublicationYear "2011" @default.
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