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- W2376582063 abstract "Objective:To investigate the apoptosis and its possible mechanism of human A549 cells induced by argon-helium-based CRYOcare System in vitro.Methods:The A549 cells cultured on the cover glass were subject to cryo-treatment via CRYOcare Cryosurgical Unit.Then cell apoptosis was examined with terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL) and DNA gel electrophores.The expression of bcl-2 protein and bax protein was detected by immunohistochemical procedures.Results:In the periphery of the cryolesion,apopotic cells can be determined by morphological analysis,TUNEL staining and DNA ladder.The expression of bax protein increased significantly,meanwhile the expression of bcl-2 protein had no obvious change.Conclusion:The argon-helium-based CRYOcare System can induce cell apoptosis in the periphery of the cryolesion by upregulation of bax expression but not bcl-2 expression.And chemotherapy or radiotherapy can be combined to improve the cryosurgery efficiency." @default.
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- W2376582063 date "2007-01-01" @default.
- W2376582063 modified "2023-09-26" @default.
- W2376582063 title "Apoptosis induced by argon-helium-based CRYOcare System in human A549 cells and the expression of related genes" @default.
- W2376582063 hasPublicationYear "2007" @default.
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