Matches in SemOpenAlex for { <https://semopenalex.org/work/W2376602477> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2376602477 abstract "AIM: To construct pSUPER RNA interference expression vector targeting enhanced green fluorescent protein(EGFP) and to test the silencing effect in mammalian COS-7 cell line.METHODS: The designed oligos targeting EGFP were cloned into the pSUPER vector,which was lineated after BglⅡ and HindⅢ digestion.The recombinant vectors were confirmed by enzyme digestion analysis and DNA sequencing.The recombinant plasmids and pIRES2-EGFP were co-transfected into COS-7 cells using Lipofectamine 2000 .The suppression effect of EGFP was assayed by fluorescence microscope 48 h after transfection. RESULTS: The pSUPER siRNA expression vectors were constructed and confirmed after the enzyme digestion analysis and the DNA sequencing.The over-expression of EGFP was detected in the COS-7 cells which were transfected pIRES2-EGFP or co-transfected pIRES2-EGFP and pSUPER.In comparison with that of the control group,the expression of EGFP in COS-7 cells which were co-transfected pIRES2-EGFP and pSUPER-R237 or pSUPER-R304 or pSUPER-R447 was weaker and the number of cells emitting green fluorescence decreased distinctly.CONCLUSION: pSUPER siRNA expression vectors targeting EGFP are successfully constructed.The expression of EGFP gene is inhibited effectively in mammalian cells which are co- transfected with pIRES2-EGFP and pSUPER-Rs." @default.
- W2376602477 created "2016-06-24" @default.
- W2376602477 creator A5044918092 @default.
- W2376602477 date "2005-01-01" @default.
- W2376602477 modified "2023-09-28" @default.
- W2376602477 title "Construction and identification of RNA interference expression vector targeting EGFP" @default.
- W2376602477 hasPublicationYear "2005" @default.
- W2376602477 type Work @default.
- W2376602477 sameAs 2376602477 @default.
- W2376602477 citedByCount "0" @default.
- W2376602477 crossrefType "journal-article" @default.
- W2376602477 hasAuthorship W2376602477A5044918092 @default.
- W2376602477 hasConcept C104317684 @default.
- W2376602477 hasConcept C119056186 @default.
- W2376602477 hasConcept C129312508 @default.
- W2376602477 hasConcept C142613039 @default.
- W2376602477 hasConcept C153911025 @default.
- W2376602477 hasConcept C166703698 @default.
- W2376602477 hasConcept C174510640 @default.
- W2376602477 hasConcept C22744801 @default.
- W2376602477 hasConcept C33161422 @default.
- W2376602477 hasConcept C40767141 @default.
- W2376602477 hasConcept C49923114 @default.
- W2376602477 hasConcept C54009773 @default.
- W2376602477 hasConcept C54355233 @default.
- W2376602477 hasConcept C552990157 @default.
- W2376602477 hasConcept C67705224 @default.
- W2376602477 hasConcept C81885089 @default.
- W2376602477 hasConcept C86803240 @default.
- W2376602477 hasConcept C92087593 @default.
- W2376602477 hasConceptScore W2376602477C104317684 @default.
- W2376602477 hasConceptScore W2376602477C119056186 @default.
- W2376602477 hasConceptScore W2376602477C129312508 @default.
- W2376602477 hasConceptScore W2376602477C142613039 @default.
- W2376602477 hasConceptScore W2376602477C153911025 @default.
- W2376602477 hasConceptScore W2376602477C166703698 @default.
- W2376602477 hasConceptScore W2376602477C174510640 @default.
- W2376602477 hasConceptScore W2376602477C22744801 @default.
- W2376602477 hasConceptScore W2376602477C33161422 @default.
- W2376602477 hasConceptScore W2376602477C40767141 @default.
- W2376602477 hasConceptScore W2376602477C49923114 @default.
- W2376602477 hasConceptScore W2376602477C54009773 @default.
- W2376602477 hasConceptScore W2376602477C54355233 @default.
- W2376602477 hasConceptScore W2376602477C552990157 @default.
- W2376602477 hasConceptScore W2376602477C67705224 @default.
- W2376602477 hasConceptScore W2376602477C81885089 @default.
- W2376602477 hasConceptScore W2376602477C86803240 @default.
- W2376602477 hasConceptScore W2376602477C92087593 @default.
- W2376602477 hasLocation W23766024771 @default.
- W2376602477 hasOpenAccess W2376602477 @default.
- W2376602477 hasPrimaryLocation W23766024771 @default.
- W2376602477 hasRelatedWork W2107497648 @default.
- W2376602477 hasRelatedWork W2141702622 @default.
- W2376602477 hasRelatedWork W2352352426 @default.
- W2376602477 hasRelatedWork W2353286559 @default.
- W2376602477 hasRelatedWork W2354775851 @default.
- W2376602477 hasRelatedWork W2356443183 @default.
- W2376602477 hasRelatedWork W2357964561 @default.
- W2376602477 hasRelatedWork W2358891865 @default.
- W2376602477 hasRelatedWork W2369519202 @default.
- W2376602477 hasRelatedWork W2370656951 @default.
- W2376602477 hasRelatedWork W2371028254 @default.
- W2376602477 hasRelatedWork W2381116097 @default.
- W2376602477 hasRelatedWork W2384147157 @default.
- W2376602477 hasRelatedWork W2385006089 @default.
- W2376602477 hasRelatedWork W2387614848 @default.
- W2376602477 hasRelatedWork W2388606850 @default.
- W2376602477 hasRelatedWork W2389157526 @default.
- W2376602477 hasRelatedWork W2390491244 @default.
- W2376602477 hasRelatedWork W2414930076 @default.
- W2376602477 hasRelatedWork W650843890 @default.
- W2376602477 isParatext "false" @default.
- W2376602477 isRetracted "false" @default.
- W2376602477 magId "2376602477" @default.
- W2376602477 workType "article" @default.