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- W2376610500 abstract "Objective To establish a method to rapidly detect and identify pathogens in CSF with universal primers PCR and restriction enzyme analysis. Methods According to the analysis of the conservative and variable regions in bacterial 16S rRNA genes, a universal primer was designed for all bacteria capable of amplifying DNA of various bacteria to determine whether there were bacteria. The PCR products were then digested with different restriction enzymes to finally identify clinical pathogens in 102 specimens of CSF. The results were compared with common culture method. Results The detection limits were 8 cfu·mL -1 for E.coli and 250 cfu·mL -1 for S.aureus. A comparison of 102 CSF samples detected by both UP PCR combined with RFLP and conventional culture method revealed the sensitivity, specificity, positive and negative values of 92.3%,96.6%,80.0% and 98.0%, respectively, for UP PCR and RFLP. Conclusion The universal PCR coupled with restriction enzyme analysis can rapidly detect and identify bacterial pathogens in CSF." @default.
- W2376610500 created "2016-06-24" @default.
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- W2376610500 date "2003-01-01" @default.
- W2376610500 modified "2023-09-24" @default.
- W2376610500 title "RAPID DETECTION AND IDENTIFICATION OF PATHOGENS IN CSF WITH UNIVERSAL PRIMERS PCR AND RFLP" @default.
- W2376610500 hasPublicationYear "2003" @default.
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