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- W2376655337 abstract "The aim was to detect the genetic variation of Paragonimus in 5 areas in Zhejiang Province and Fujian Province with technologies of SSR-PCR and normal PCR,so as to compare the differences on the gene horizontal classification.Our method was to amplify the genome DNA with the technology of simple sequence repeat-anchored PCR(SSR-PCR),and count the genetic distance with the method of Nei,and construct the dendrogram with SPSS software.This study was also corduct to amplify COI gene and ITS2 gene by normal PCR,analyze homology through BoiEdit,and construct stammbaum through MEGA.The SSR-PCR product of the Paragonimus speciman in different areas was polymorphic.There were obvious differences between Jinhua in Zhejiang Province,Yongjia in Zhejiang Province,Zhouning in Fujian Province and pinghe and zhenghe in Fujian province.The genetic distance of the first two is the nearest(0.3333),and the specimen in Jinhua is the farthest to the Pinghe,with each other(0.8667).According to the normal PCR gene sequencing,specimen in Jinhua,Yongjia,and Zhouning are near in the phylogenetic tree,and the specimen in Jinhua is nearest to that in Yongjia.The results are similar on genetic variation of Paragonimus with technologies of SSR-PCR and normal PCR.It's indicated that SSR-PCR was a more convenient method on studying the genetic variation of Paragonimus." @default.
- W2376655337 created "2016-06-24" @default.
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- W2376655337 date "2011-01-01" @default.
- W2376655337 modified "2023-09-28" @default.
- W2376655337 title "A comparison between SSR-PCR and normal PCR technology in detection of Paragonimus in different areas" @default.
- W2376655337 hasPublicationYear "2011" @default.
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