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- W2376656122 abstract "Objective To explore the effect of postasphyxial-serum in neonate on the expressions of Bcl-2-antagonist of cell death(BAD)and Bcl-2-associated X protein(BAX)in renal tubular cells(HK-2).Methods HK-2 cells were used as target cells.The experiment were divided into control group,asphyxia group and pyrrolodine dithiocarbamate(PDTC)blocking group.Control group:DMEM culture fluid was not contained asphyxia blood serum in every group;asphxia group:DMEM culture fluid contained 20 mL/L asphyxia blood serum in every group;PDTC blocking group:DMEM culture fluid contained 20 mL/L asphyxia blood serum and 40 μmol/L PDTC in every group.The expressions of both BAD and BAX on cytoplast were detected by immunohistochemical method.Results Calculated Points according to HSCORE,compared with controls group[(1.97±0.26)and(1.77±0.11)],after stimulated with postasphyxial-serum,the expressions of both BAD and BAX of HK-2 cells of asphyxia group[(2.73±0.20)and(2.44±0.13)] and PDTC blocking group[(2.38±0.13)and(2.17±0.08)] significantly increased[F(BAD)=28.61,F(BAX)=15.51 Pa0.01],but compared with asphyxia group,the expressions of both BAD and BAX of PDTC blocking group were significantly decreased(Pa0.01).Conclusions Postasphyxial-serum is a stimulus that can induce the expression of both BAD and BAX in HK-2 and its intracellular signal transduction mechanism is presumably involved in the activation of nuclear factor-κB." @default.
- W2376656122 created "2016-06-24" @default.
- W2376656122 creator A5076812378 @default.
- W2376656122 date "2008-01-01" @default.
- W2376656122 modified "2023-09-23" @default.
- W2376656122 title "Effect of Postasphyxial-Serum in Neonate on the Expressions of Bcl-2-Antagonist of Cell Death and Bcl-2-Associated X Protein in Renal Tubular Cells" @default.
- W2376656122 hasPublicationYear "2008" @default.
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