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- W2376732018 abstract "Objective:To construct small hairpin(shRNA) vector targeting Skp2 and p27 and investigate its effect on the expressions of Skp2 and p27 as well as on the growth of lung cancer cells.Methods:The specific shRNA sequence was designed,synthesized and cloned into plasmid pGenesil-1.The lung cancer cells were selected by G418 screening.The mRNA and protein expressions of Skp2 and p27 were analyzed by RT-PCR and Western blotting.Cell proliferation was detected by MTT assay and cell growth curve was drawn to analyze the inhibitory effects on cell proliferation.Results:The Skp2 mRNA and protein expression was significantly decreased after transfection of interfering plasmid.p27 protein level was significantly increased,which resulted from decrease in p27 degradation caused by inhibition of Skp2 expression.The cell growth curves indicated that the proliferation of cells was inhibited significantly compared with the contro1 group.Conclusion:The shRNA vector targeting Skp2 gene effectively inhibited the proliferation of lung cancer cells,down-regulated Skp2 gene expression,and up-regulated p27 gene expression.The method laid the an experimental basis for lung cancer gene therapy." @default.
- W2376732018 created "2016-06-24" @default.
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- W2376732018 date "2008-01-01" @default.
- W2376732018 modified "2023-09-23" @default.
- W2376732018 title "Effects of shRNA interference on the expressions of Skp2 and p27 as well as the growth of lung cancer cells" @default.
- W2376732018 hasPublicationYear "2008" @default.
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