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- W2376733705 abstract "In order to investigate the application values of polymerase chain reaction(PCR) technique in detection of pathogenic bacteria in surface water,the universal primers were designed and synthesized according to the high conversation of 16S rRNA gene.The DNA of bacteria from several water bodies and secondary effluent in the urban area of Xi'an City were extracted and then amplified with the primers,and sequencing and homology analyses of the PCR production were conducted.As a result,the PCR products of the 4 reference strains were identified at 320 bp on the electrophoregrams,and they were further verified by sequencing and homology analyses.The detection limit of the total DNA of E.coli reference strain was about 250(ng/L) and the sensibility of PCR detection was up to 0.275(CFU/mL).Regarding different water samples,limpid electrophoresis strips were identified at 320 bp for 1 river and 2 lakes in the urban area,as well as the secondary effluent from a domestic wastewater treatment plant,all of which are believed to be under the influence of domestic pollution,while no strip was identified for another river water which is the well protected source for potable water supply.Bacteria enumeration was also conducted by conventional culture method for comparison.Regarding fecal coliform,it was only detected from the secondary effluent and a polluted lake." @default.
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- W2376733705 date "2007-01-01" @default.
- W2376733705 modified "2023-09-23" @default.
- W2376733705 title "Application of Pathogenic Bacteria Detection in Surface Water by Universal Primer PCR Method" @default.
- W2376733705 hasPublicationYear "2007" @default.
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