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- W2376739884 abstract "[Objective] To establish a TaqMan real-time PCR for rapid detection of Vibrio parahemolyticus(VP) in food poisoning and food contamination. [Methods] A set of primers was designed based on the sequence of tdh gene published in GenBank to detect Vibrio parahemolyticus. The corresponding TaqMan probe labeled with FAM was designed. The TaqMan real-time PCR assay was applied to detect Vibrio parahemolyticus in food. [Results] The sensitivity of the established method was 1.3×104cfu /ml(33cfu /PCR reaction) . There was no cross-reaction with other tested bacteria. [Conclusion] The established TaqMan real-time PCR assay is rapid,sensitive and specific. It could be applied to the rapid diagnosis of Vibrio parahemolyticus food poisoning and investigation of the contamination status of it in food." @default.
- W2376739884 created "2016-06-24" @default.
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- W2376739884 date "2008-01-01" @default.
- W2376739884 modified "2023-09-27" @default.
- W2376739884 title "RAPID DETECTION OF VIBRIO PARAHEMOLYTICUS BY TAQMAN AND REAL-TIME PCR" @default.
- W2376739884 hasPublicationYear "2008" @default.
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