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- W2376747511 abstract "Based on the immunologic character of Pichia pastoris yeast, a new therapeutic vaccine, whole recombinant yeast, was used to explore a new way to activate cell-mediated anti-viral immunity.The recombinant plasmids, pPIC9K/S and PIC9K/hsp(1-370)-S, were constructed by inserting the gene encoding HBsAg, HSP70 (1-370) -HBsAg into vector pPIC9K and then the recombinants were transfected into Pichia pastoris yeast,GS115, respectively. Then that recombinant yeast immunized BALB/C mice were detected for humoral and cellular immunity to HBsAg.Recombinant yeast successfully activated the humoral immunity to HBsAg in mice, but failed to activate the cellular immunity.The whole recombinant yeast can be used as vaccine, but need further study for optimal way of immunization." @default.
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- W2376747511 date "2003-12-01" @default.
- W2376747511 modified "2023-09-23" @default.
- W2376747511 title "[The primary study on the anti-HBV effect of whole recombinant yeast]." @default.
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