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- W2376750845 abstract "Objective To study the method of collagen gel immobilization culture of rat hepatocytes and to observe the function and morphological characteristics of the cultured hepatocytes. Methods Rats hepatocytes were isolated by two step perfusion and were seeded onto dishes mixed up with rat tail collagen I solution. After the collagen turned into gel form, the hepatocytes were cultured with routine culturing method. The urea(BUN) and Lactate dehydrogenase(LDH) content of the culture media and the cellular morphologies of hepatocytes in the collagen gel were observed with phase contrast photomicrographs. Results Rats hepatocytes were cultured in the collagen gel success fully. During the cultural process, the high secretion of BUN and the low leakage of LDH of the rat's hepatocytes could all be estimated. The typical cellular morphological characteristics of cultured hepatocytes could also be observed.Conclusions The collagen gel immobilization culture can provide an environment that is more closely resembing that in vivo, which maybe more eligible for use in the study of bioartificial liver." @default.
- W2376750845 created "2016-06-24" @default.
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- W2376750845 date "2003-01-01" @default.
- W2376750845 modified "2023-09-24" @default.
- W2376750845 title "Collagen-gel immobilization culture of rat hepatocytes." @default.
- W2376750845 hasPublicationYear "2003" @default.
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