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- W2376768501 abstract "Objective:To develop a RP-HPLC method for the simultaneous determination of three flavonoids(myricetin,quercetin,kaempferol) in pine needles of Cedrus deodara.Methods: The samples were pretreated,including reflux extraction with 60% methanol for 1 h(with the ratio of liquor to material of 50∶ 1),and then extracting solution hydrolysis with 10% hydrochloric acid for another 1 h.Agilent TC-C18(250 mm×4.6 mm,5 μm) column was adopted;The mobile phase was acetonitrile-0.1% phosphoric acid solution with the gradient elution at a flow rate of 1.0 mL·min-1;The column temperature was 30 ℃ and the detection wavelength was 360 nm.Results: Good linear correlations were found in the ranges of 0.4-8.0 μg·mL-1 for myricetin,0.5-9.5 μg·mL-1 for quercetin and 0.8-15.2 μg·mL-1 for kaempferol,respectively.The correlation coefficients were between 0.9998-0.9999.The average recoveries(n=9) of the three flavonoids were 98.4%,97.5% and 98.9%,respectively.Conclusion: The method is simple,rapid and efficient,which is applicable for determination of flavonoids in pine needles of C.deodara." @default.
- W2376768501 created "2016-06-24" @default.
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- W2376768501 date "2012-01-01" @default.
- W2376768501 modified "2023-09-23" @default.
- W2376768501 title "Simultaneous determination of myricetin,quercetin and kaempferol in pine needles of Cedrus deodara by RP-HPLC" @default.
- W2376768501 hasPublicationYear "2012" @default.
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