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- W2376774328 abstract "Aim:To synthesize gene and construct prokaryotic expression vector of hepatitis B core antigen(HBcAg).Methods:By selecting the biased codon in E.coli,the HBcAg gene was optimized by software of Synthetic Gene Designer and was divided into 24 oligonucleotide fragments.The complete target fragment was synthesized using assembly PCR and cloned into pET30a(+)vector at NdeⅠ/XhoⅠ site.After it was analyzed with restriction endonuclease digestion and DNA sequencing analysis,the gene which contained error was corrected by site-directed mutagenesis.Then pET30a(+)-HBcAg was induced with IPTG.Finally,the expression product was identified with SDS-PAGE and Western Blot.Results:The recombinant plasmid of pET30a(+)-HBcAg were generated.The target protein existed in form of inclusion body was expressed highly in E.coli,and the antigenicity of the protein was specific.Conclusion:The synthesis of HBcAg gene and construction of prokaryotic expression vector were successful." @default.
- W2376774328 created "2016-06-24" @default.
- W2376774328 creator A5040027560 @default.
- W2376774328 date "2009-01-01" @default.
- W2376774328 modified "2023-09-23" @default.
- W2376774328 title "Gene synthesis and construction of prokaryotic expression vector of hepatitis B core antigen" @default.
- W2376774328 hasPublicationYear "2009" @default.
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