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- W2376806676 abstract "Objective To establish a simple,efficient and stable method of primary neurons' culture and transfection. Methods The hippocampus of SD rats within 2 days after birth was separated under aseptic condition,which was digested and received repeated deposition to gain the hippocampus cell suspension. The neurons were purified by differential adhension method and were cultured with serum-free medium. The morphological changes of neurons were observed by inverted microscope. Microtubule-associated protein( MAP2)antibodies were used to identify the cells. The lentiviral transfected neurons were used to observe the efficiency of neuron transfection.Results The dense networks of primary neurons,which were cultured in vitro,were formed 10 to 15 days after culture,with healthy cells and specific characteristics of the neurons. The purity of neurons was more than 95% identified by MAP2. The transfection efficiency achieved more than 95% using lentiviral transfected neurons. Conclusion The method we have used to culture primary neurons can be adopted to obtain highly purified neurons. The lentiviral is an excellent carrier for neuron transfection." @default.
- W2376806676 created "2016-06-24" @default.
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- W2376806676 date "2015-01-01" @default.
- W2376806676 modified "2023-09-23" @default.
- W2376806676 title "Culture and lentiviral transfection of primary neurons in vitro" @default.
- W2376806676 hasPublicationYear "2015" @default.
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