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- W2376836925 abstract "In order to develop an efficient protein extraction method suitable for two-dimensional electrophoresis from tea plant,three methods(TCA/acetone precipitation,improved Tris-HCl extraction and phenol-methanol/ammonium acetate precipitation) for total protein extraction in plant were compared.The result showed that the improved Tris-HCl extraction was the best method suitable for tea leaf protein sample preparation in these three techniques and the extracts were separated by two-dimensional electrophoresis.Consequently,1117±9.89 protein spots were found and their isoelectric points were found to lie between pI5～7.The relative molecular weight of proteins was between 15.00～95.00kD.The improved Tris-HCl extraction could get the highest resolution of two dimensional electrophoresis and high yield of protein,and it could meet the requirement for the research on tea proteomecis." @default.
- W2376836925 created "2016-06-24" @default.
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- W2376836925 date "2011-01-01" @default.
- W2376836925 modified "2023-09-24" @default.
- W2376836925 title "Protein Extraction Method Suitable for Tea Plant" @default.
- W2376836925 hasPublicationYear "2011" @default.
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