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- W2376875487 abstract "Objective To screen the immunodominant antigens of secretory proteins from Aspergillus fumigatus by immunoproteomic in order to serve as the marker of clinical diagnosis and effectiveness of therapy for invasive aspergillosis.Methods The proteins secreted by Aspergillus fumigatus which grew in YEPG media for 14 days were separated by two-dimensional PAGE(2-DE),and then transferred to PVDF membranes.Immunoblot was performed by using the mixed sera from definitely diagnostic patients with invasive aspergillosis.The immunoreactive spots were identified by mass spectrometric analysis and bioinformatics method.Results 2-DE immunobloting showed that the sera from invasive aspergillosis patients reacted strongly with a series of antigens in the secretory proteins.Forty dots were recognized by sera of patients and were analyzed by mass spectrometry.Among them,39 immunoreactive spots were successfully identified,which were belonged to 17 corresponding proteins,including dipeptidyl peptidase,NAD-dependent malate dehydrogenase,thioredoxin reductase,aldehyde dehydrogenase,fructose-bisphosphate aldolase,3-hydroxybutyryl-CoA dehydrogenase,etc.In the 17 proteins,2 were reported before and 15 were newly reportedimmunogenetic antigens.Seven immunogenic proteins displayed multiple spots.The novel immunogenic protein No.2,which contained 13 spots,showed the highest immunoreactivity and was identified as thioredoxin reductase.Conclusion The immunoproteomic technology should be effective approach for screening immunodominant antigens of pathogens.The antigens identified in this study might serve as the potential markers for the infection of Aspergillus fumigatus,but the diagnostic values have to be further evaluated." @default.
- W2376875487 created "2016-06-24" @default.
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- W2376875487 date "2011-01-01" @default.
- W2376875487 modified "2023-09-23" @default.
- W2376875487 title "Immunodominant antigen screening of secretory proteins from Aspergillus fumigatus by immunoproteomics" @default.
- W2376875487 hasPublicationYear "2011" @default.
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