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- W2376880499 abstract "Universal primers for 16S rRNA gene was used to detect phytoplasmas in sunshine tree( Cassia surattensis )showing witches'-broom symptom. A 1.2 kb DNA fragment was amplified by nested-PCR. The result indicated existence of phytoplasma associated with this diseased plant. The amplified fragment was ligated into pGEM-T easy vector and transformed into the competent cell of E.coli JM109 strain. Cloned DNA fragments were verified by PCR,sequencing and phylogenetic analysis. The results showed that the content of G+C in the sequenced 16S rDNA was 45.8%. This strain (STWB) shared highest homology (99.4%) with phytoplasma strains in Elm yellows (16SrV group) but is obviously under 97.0% with other groups. So the results made it clear that this phytoplasma strain is one of the members of Elm yellows phytoplasma group." @default.
- W2376880499 created "2016-06-24" @default.
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- W2376880499 date "2005-01-01" @default.
- W2376880499 modified "2023-09-23" @default.
- W2376880499 title "Detection and identification of phytoplasma association with sunshine tree witches'-broom" @default.
- W2376880499 hasPublicationYear "2005" @default.
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