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- W2376926760 abstract "Objective To explore the effect of interphase fluorescence in situ hybridization(FISH) technique in detecting of bcr-abl fusion gene in Ph~-bcr-abl~+ hematological disorders.Methods bcr-abl du- al-color and dual-fusion fluorescence in situ hybridization(D-FISH)technique was carried out to detect bcr- abl fusion gene in DNA level in 7 patients with hematological disorders who were Ph(-)or indefinite by con- ventional cytogeneties(CC)and bcr-abl(+)by nested-reverse transcriptase polymerase chain reaction(nested- RT-PCR).Results 2 specimens from case 2 were investigated by FISH and showed 11.5% and 13.5% bcr- abl(+)cells respectively.Case 4 and case 6 showed 1% bcr-abl(+)cells respectively by FISH,slightly higher than normal cut-off value.Case 7 showed 0.5% bcr-abl(+)cells and the others didn't show bcr-abl(+)cells by FISH.Conclusion Ph~-bcr-abl~+ patients with hematological disorders have low level bcr-abl(+)cells in DNA level.FISH is a useful method to detect bcr-abl(+)cells from patient with Ph(-)or indefinite or whose bcr or abl breakpoints is not in common breakpoints." @default.
- W2376926760 created "2016-06-24" @default.
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- W2376926760 date "2007-01-01" @default.
- W2376926760 modified "2023-09-23" @default.
- W2376926760 title "Detection of bcr-abl fusion gene in Ph~- bcr-abl~+ hematological disorders by fluorescence in situ hybridization" @default.
- W2376926760 hasPublicationYear "2007" @default.
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