Matches in SemOpenAlex for { <https://semopenalex.org/work/W2376932064> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2376932064 abstract "Objective To establish a method to investigate whether flow cytometry detection of minimal residual disease(MRD) in bone marrow(BM) could predict prognosis and clinically guide the risk-adjusted therapy for neuroblastoma(NB) patients. Methods Human TGW NB cell line was used to detect the immunophenotype of NB cells by flow cytometry (FCM). The FCM sensitivity of the cocktail of CD45~ FITC /CD81~ PE /CD56~ PECy5+ was tested in our lab. The results of MRD with FCM were used to evaluate neuroblastoma metastasis in BM at diagnosis and during chemotherapy.Results The TGW cell line expressed CD56 and CD81 antigen and did not express CD45. The FCM sensitivity in our lab was 1/10~ 4 .②In 144 samples from 61 patients, NB cells were found in 23 of them morphologically(BM smear). All of those 23 samples were CD45~-/ CD81~+/ CD56~+ positive by FCM. Thirty-nine BM samples were cytologically negative by BM smear but CD45~-/ CD81~+/ CD56~+ positive by FCM. There was statistically significant difference between the two methods (P﹤0.01). ③ BM samples from 31 patients were positive for neuroblastoma by FCM at diagnosis, eleven of them became negative after average 4 courses of chemotherapy. All of those 11 patients remained alive without evidence of disease (median23mos,range8-38 months). In contrast, twenty patients whose BM samples were still positive, when they received the same chemotherapy, eleven of them were relapsed and 1 patient was died of disease(median17.5 months,range6-48 months). There was statistically significant difference in disease-free survival between the two groups(P﹤0.01).④MRD in BM was tested by FCM before PBSC harvest for 19 advanced neuroblastoma patients. Fourtheen was negative, two of them were relapsed. Eleven patients remained alive without evidence of disease (median 9 months after transplant,range 4-30 months). In contrast, other 5 patients whose BM remained positive before PBSC harvest, all of them were relapsed(median 10.5months after transplant,range 1-21 months). There was a statistically significant difference in disease-free survival after transplant between the two groups(P﹤0.05).Conclusions The method for detecting micrometastasis of NB in BM or peripheral blood using FCM was high sensitive and specific. It could evaluate the response of treatment by monitoring the MRD of BM. Positive NB MRD in BM during therapy was an unfavourable factor of prognosis." @default.
- W2376932064 created "2016-06-24" @default.
- W2376932064 creator A5014575743 @default.
- W2376932064 date "2006-01-01" @default.
- W2376932064 modified "2023-09-26" @default.
- W2376932064 title "The detection of minimal residual disease with flow cytometry and the relationship between prognosis and minimal residual disease in bone marrow in advanced neuroblastoma" @default.
- W2376932064 hasPublicationYear "2006" @default.
- W2376932064 type Work @default.
- W2376932064 sameAs 2376932064 @default.
- W2376932064 citedByCount "0" @default.
- W2376932064 crossrefType "journal-article" @default.
- W2376932064 hasAuthorship W2376932064A5014575743 @default.
- W2376932064 hasConcept C126322002 @default.
- W2376932064 hasConcept C142724271 @default.
- W2376932064 hasConcept C143998085 @default.
- W2376932064 hasConcept C196166836 @default.
- W2376932064 hasConcept C203014093 @default.
- W2376932064 hasConcept C2776694085 @default.
- W2376932064 hasConcept C2776715637 @default.
- W2376932064 hasConcept C2779823535 @default.
- W2376932064 hasConcept C2780007613 @default.
- W2376932064 hasConcept C54355233 @default.
- W2376932064 hasConcept C553184892 @default.
- W2376932064 hasConcept C71924100 @default.
- W2376932064 hasConcept C81885089 @default.
- W2376932064 hasConcept C86803240 @default.
- W2376932064 hasConcept C90924648 @default.
- W2376932064 hasConceptScore W2376932064C126322002 @default.
- W2376932064 hasConceptScore W2376932064C142724271 @default.
- W2376932064 hasConceptScore W2376932064C143998085 @default.
- W2376932064 hasConceptScore W2376932064C196166836 @default.
- W2376932064 hasConceptScore W2376932064C203014093 @default.
- W2376932064 hasConceptScore W2376932064C2776694085 @default.
- W2376932064 hasConceptScore W2376932064C2776715637 @default.
- W2376932064 hasConceptScore W2376932064C2779823535 @default.
- W2376932064 hasConceptScore W2376932064C2780007613 @default.
- W2376932064 hasConceptScore W2376932064C54355233 @default.
- W2376932064 hasConceptScore W2376932064C553184892 @default.
- W2376932064 hasConceptScore W2376932064C71924100 @default.
- W2376932064 hasConceptScore W2376932064C81885089 @default.
- W2376932064 hasConceptScore W2376932064C86803240 @default.
- W2376932064 hasConceptScore W2376932064C90924648 @default.
- W2376932064 hasLocation W23769320641 @default.
- W2376932064 hasOpenAccess W2376932064 @default.
- W2376932064 hasPrimaryLocation W23769320641 @default.
- W2376932064 hasRelatedWork W177056526 @default.
- W2376932064 hasRelatedWork W1981653011 @default.
- W2376932064 hasRelatedWork W206898877 @default.
- W2376932064 hasRelatedWork W2538309525 @default.
- W2376932064 hasRelatedWork W2557927945 @default.
- W2376932064 hasRelatedWork W2558425632 @default.
- W2376932064 hasRelatedWork W2573207423 @default.
- W2376932064 hasRelatedWork W2586059224 @default.
- W2376932064 hasRelatedWork W2586200752 @default.
- W2376932064 hasRelatedWork W2586483048 @default.
- W2376932064 hasRelatedWork W2593163018 @default.
- W2376932064 hasRelatedWork W2609771959 @default.
- W2376932064 hasRelatedWork W2625814291 @default.
- W2376932064 hasRelatedWork W2883912791 @default.
- W2376932064 hasRelatedWork W2966592462 @default.
- W2376932064 hasRelatedWork W3030060885 @default.
- W2376932064 hasRelatedWork W3030693636 @default.
- W2376932064 hasRelatedWork W3122079021 @default.
- W2376932064 hasRelatedWork W3196794900 @default.
- W2376932064 hasRelatedWork W3205287400 @default.
- W2376932064 isParatext "false" @default.
- W2376932064 isRetracted "false" @default.
- W2376932064 magId "2376932064" @default.
- W2376932064 workType "article" @default.