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- W2376949572 abstract "Objective To express the responsive protein CrdS of Helicobacter pylori in prokaryotic cells,analyze its bioinformatics and investigate its regulatory mechanism on acidic adaptation of H.pylori.Methods The hp1364 gene encoding CrdS protein was amplified by PCR from whole genomic DNA of H.pylori standard strain 26695,and inserted into prokaryotic expression vector pQE30.The constructed recombinant plasmid pQE30-hp1364 was transformed to E.coli XL1-blue for expression under induction of IPTG.The expressed recombinant CrdS protein was identified by Western blot and analyzed for bioinformatics.Results Restriction analysis and sequencing proved that recombinant plasmid pQE30hp1364 was constructed correctly.The expressed recombinant protein,with a relative molecular mass of about 46 000,contained about 30% of total somatic protein,mainly existed in a form of inclusion body,and showed specific binding to rabbit anti-His-tag polyclonal antibody.Bioinformatic analysis showed high homologies of nucleotide and amino acid sequences of CrdS to those of other H.pylori strains(HpG27,F30 and B38).Many hydrophilic regions were presented on the surface of CrdS,which contained several kinds of amino acids easily to be phosphorylated.Conclusion The CrdS protein of H.pylori was expressed successfully in prokaryotic cells and analyzed for bioinformatics,which provided an experimental material for further study on responding mechanism of CrdS as well as the route in resist H.pylori infection through blocking signal response." @default.
- W2376949572 created "2016-06-24" @default.
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- W2376949572 date "2013-01-01" @default.
- W2376949572 modified "2023-09-23" @default.
- W2376949572 title "Prokaryotic expression and bioinformatics of responsive protein CrdS of Helicobacter pylori" @default.
- W2376949572 hasPublicationYear "2013" @default.
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