FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2376957648> ?p ?o ?g. }

• W2376957648 endingPage "2970" @default.
• W2376957648 startingPage "2966" @default.
• W2376957648 abstract "Objective To investigate the effects of matrix metalloproteinase-9 (MMP-9) on granulocyte colony stimulation factor (G-CSF)-induced hematopoietic stem/progenitor cell (HSPC) mobilization in healthy donors of hematopoietic stem cells. Methods Peripheral blood (PB) samples and bone marrow (BM) blood samples were collected from 12 healthy donors of hematopoietic stem cell before and 5 days after G-CSF-induced mobilization. CD34+ cells were isolated and purified. ELISA was used to detect the protein expression of MMP-9 in the peripheral blood and BM blood of the healthy donors. The protein expression of MMP-9 in the BM blood was detected by ELISA and immunohistochemistry, and the stromal cell-derived factor-1 (SDF-1) level in the BM blood was detected by ELISA. The mRNA expression of MMP-9 in the BM blood samples was detected by RT-PCR. HT1080 cells rich in MMP-9 were cultured. CD34+ cells were co-cultured with the supernatant of HT1080 cell culture fluid. CD34+ cells cultured in Iscove′s modified Dulbecco′s medium were used as control group. Fluorescence-activeted cell sorter was used to detect the CXCR4 expression on the suface of the CD34+ cells. In the transwell experiment CD34+ cells were divided into 4 groups: control group, o-phenanthroline (MMP-9 chemical inhibitor , MPI) group, HT1080 sup group, and HT1080+MPI group to be co-cultured with buffer, o-phenanthroline, supernatant of culture fluid of HT1080 cells, or supernatant of culture fluid of HT1080 cells Flow cytometry was used to calculate the cell migration capacity. Results The MMP-9 level of BM and PB of the healthy donors 5 days after G-CSF mobilization were 278 ng/ml±34 ng/ml and 392 ng/ml±284 ng/ml respectively, both significantly higher than those before G-CSF mobilization (42 ng/ml±17 ng/ml and 27 ng/ml±12 ng/ml respectively (P0.01 and P0.05). Western blotting showed that the SDF-1 level in the supernatant 5 days after G-CSF mobilization was 5.9 ng/ml±1.0 ng/ml, significantly lower than that before G-CSF mobilization (7.2 ng/ml±0.7 ng/ml, P0.05 ).The CXCR4 levels of the CD34+ cell from both PB and BM blood were up-regulated after co-culture with the supernatant of HT1080 cells (both P0.05). The migration capacity of CD34+ cells cultured in the supernatant of HT1080 cells was increased significantly (P0.05), however, this effect could be inhibited by MIP (P0.05). The PB WBC numbers of the G-CSF group and G-CSF+MPI group were 14.9×106/L±4.3×106/L and 12.3×106/L±1.2×106/L respectively, the PB WBC numbers of the G-CSF+MPI group was significantly lower than that of the G-CSF group (P0.05), however, significantly higher than that of the negative control group (6.8×106/L±2.5×106/L, P0.05). The CFU of the G-CSF group was (84±10) U/2×105 MNC, significantly higher than that of the G-CSF+MPI group, (69±3) U/2×105 MNC (P0.05). The BM MNC number of the G-CSF group was 12.7×106/L±0.7×106/L, not significantly different from that of the G-CSF+MPI groups (13.1×106/L±1.3×106/L;P0.05). Conclusion MMP-9 probably facilitates HSPC mobilization by degrading SDF-1,up-regulating CXCR4 expression on the CD34+ cells, and increasing the migration ability of CD34+ cells." @default.
• W2376957648 created "2016-06-24" @default.
• W2376957648 creator A5006292484 @default.
• W2376957648 creator A5013558745 @default.
• W2376957648 creator A5026627692 @default.
• W2376957648 creator A5041332463 @default.
• W2376957648 creator A5047470425 @default.
• W2376957648 creator A5056202706 @default.
• W2376957648 creator A5077458512 @default.
• W2376957648 creator A5086844687 @default.
• W2376957648 date "2006-11-14" @default.
• W2376957648 modified "2023-09-24" @default.
• W2376957648 title "[The effect of matrix metalloproteinase-9 in granulocyte colony stimulation factor-induced stem cell mobilization]." @default.
• W2376957648 doi "https://doi.org/10.3760/j:issn:0376-2491.2006.42.005" @default.
• W2376957648 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17288807" @default.
• W2376957648 hasPublicationYear "2006" @default.
• W2376957648 type Work @default.
• W2376957648 sameAs 2376957648 @default.
• W2376957648 citedByCount "2" @default.
• W2376957648 countsByYear W23769576482012 @default.
• W2376957648 crossrefType "journal-article" @default.
• W2376957648 hasAuthorship W2376957648A5006292484 @default.
• W2376957648 hasAuthorship W2376957648A5013558745 @default.
• W2376957648 hasAuthorship W2376957648A5026627692 @default.
• W2376957648 hasAuthorship W2376957648A5041332463 @default.
• W2376957648 hasAuthorship W2376957648A5047470425 @default.
• W2376957648 hasAuthorship W2376957648A5056202706 @default.
• W2376957648 hasAuthorship W2376957648A5077458512 @default.
• W2376957648 hasAuthorship W2376957648A5086844687 @default.
• W2376957648 hasConcept C10205521 @default.
• W2376957648 hasConcept C109159458 @default.
• W2376957648 hasConcept C1491633281 @default.
• W2376957648 hasConcept C153911025 @default.
• W2376957648 hasConcept C16685009 @default.
• W2376957648 hasConcept C16930146 @default.
• W2376957648 hasConcept C185592680 @default.
• W2376957648 hasConcept C201750760 @default.
• W2376957648 hasConcept C2022786 @default.
• W2376957648 hasConcept C203014093 @default.
• W2376957648 hasConcept C2777751087 @default.
• W2376957648 hasConcept C2777983669 @default.
• W2376957648 hasConcept C2780007613 @default.
• W2376957648 hasConcept C28328180 @default.
• W2376957648 hasConcept C502942594 @default.
• W2376957648 hasConcept C55493867 @default.
• W2376957648 hasConcept C71924100 @default.
• W2376957648 hasConcept C86803240 @default.
• W2376957648 hasConcept C95444343 @default.
• W2376957648 hasConceptScore W2376957648C10205521 @default.
• W2376957648 hasConceptScore W2376957648C109159458 @default.
• W2376957648 hasConceptScore W2376957648C1491633281 @default.
• W2376957648 hasConceptScore W2376957648C153911025 @default.
• W2376957648 hasConceptScore W2376957648C16685009 @default.
• W2376957648 hasConceptScore W2376957648C16930146 @default.
• W2376957648 hasConceptScore W2376957648C185592680 @default.
• W2376957648 hasConceptScore W2376957648C201750760 @default.
• W2376957648 hasConceptScore W2376957648C2022786 @default.
• W2376957648 hasConceptScore W2376957648C203014093 @default.
• W2376957648 hasConceptScore W2376957648C2777751087 @default.
• W2376957648 hasConceptScore W2376957648C2777983669 @default.
• W2376957648 hasConceptScore W2376957648C2780007613 @default.
• W2376957648 hasConceptScore W2376957648C28328180 @default.
• W2376957648 hasConceptScore W2376957648C502942594 @default.
• W2376957648 hasConceptScore W2376957648C55493867 @default.
• W2376957648 hasConceptScore W2376957648C71924100 @default.
• W2376957648 hasConceptScore W2376957648C86803240 @default.
• W2376957648 hasConceptScore W2376957648C95444343 @default.
• W2376957648 hasIssue "42" @default.
• W2376957648 hasLocation W23769576481 @default.
• W2376957648 hasLocation W23769576482 @default.
• W2376957648 hasOpenAccess W2376957648 @default.
• W2376957648 hasPrimaryLocation W23769576481 @default.
• W2376957648 hasRelatedWork W2015197822 @default.
• W2376957648 hasRelatedWork W2027872028 @default.
• W2376957648 hasRelatedWork W2109184076 @default.
• W2376957648 hasRelatedWork W2349920578 @default.
• W2376957648 hasRelatedWork W2355946175 @default.
• W2376957648 hasRelatedWork W2356836258 @default.
• W2376957648 hasRelatedWork W2358662456 @default.
• W2376957648 hasRelatedWork W2360020513 @default.
• W2376957648 hasRelatedWork W2363150208 @default.
• W2376957648 hasRelatedWork W2366492435 @default.
• W2376957648 hasRelatedWork W2369755944 @default.
• W2376957648 hasRelatedWork W2370208798 @default.
• W2376957648 hasRelatedWork W2385688239 @default.
• W2376957648 hasRelatedWork W2392384874 @default.
• W2376957648 hasRelatedWork W2399769825 @default.
• W2376957648 hasRelatedWork W2586442141 @default.
• W2376957648 hasRelatedWork W2587837296 @default.
• W2376957648 hasRelatedWork W3028601916 @default.
• W2376957648 hasRelatedWork W3029484904 @default.
• W2376957648 hasRelatedWork W3163993041 @default.
• W2376957648 hasVolume "86" @default.
• W2376957648 isParatext "false" @default.
• W2376957648 isRetracted "false" @default.
• W2376957648 magId "2376957648" @default.
• W2376957648 workType "article" @default.