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- W2376962572 abstract "Two bacterial strains with high chondroitinase activity were isolated from soil with transparent zones screening method.Strain 28 showed higher chondroitinase activity than other strain,which was preliminarily identified as Sphingomonas paucimobilis according to its biological characteristics and biochemical reaction performance.The optimal medium for this strain to produce chondroitinase contained 1.0% of yeast extract,0.5% of chondroitin sulfate,0.5% of NaCl,0.03% of KH2PO4 and 0.5% of MgSO4·7H2O(w/v;initial acidity pH 8.5).When 1%(v/v) of seed culture was inoculated into 40mL sterilized fermenting medium in a 250mL cotton-plugged flask and incubated at 25 ℃ for 36 h with reciprocation(100r/min),chondroitinase productivity can reach 1.2U/mL.Our findings provided an experimental basis for fermenting the bacterium to produce chondroitinase." @default.
- W2376962572 created "2016-06-24" @default.
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- W2376962572 date "2012-01-01" @default.
- W2376962572 modified "2023-09-26" @default.
- W2376962572 title "Screening and Identification of Two High Chondroitinase Producing Bacterial Strains and Determination of Their Fermenting Condition" @default.
- W2376962572 hasPublicationYear "2012" @default.
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