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- W2376963781 abstract "Objective: To clone the full-length gene of beta 2-adrenergic receptor (β2-AR).Method: Primers for PCR were designed according to the porcine β2-AR cDNA sequence in the GenBank.The β2-AR gene was amplified from the total RNA of porcine liver by RT-PCR technology,which was then cloned into pUC18 vector.The recombinant was transformed to competent cell E.coil DH5α,screening the positive clones.Result: The amplified β2-AR gene has 1257 bases,encoding 418 amino acids.Compared with the porcine β2-AR DNA sequence inscribed in GenBank, the homology is 99.52% with 99.04% amino acids identical.Conclusion: Full-length sequence of β2-AR Gene was successfully obtained,establishing foundation for gene expression and the construction of receptor-drugs screening model." @default.
- W2376963781 created "2016-06-24" @default.
- W2376963781 creator A5062435845 @default.
- W2376963781 date "2009-01-01" @default.
- W2376963781 modified "2023-09-26" @default.
- W2376963781 title "Cloning and Sequence Analysis of Beta 2-Adrenergic Receptor Gene" @default.
- W2376963781 hasPublicationYear "2009" @default.
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