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- W2376972541 abstract "Purpose In order to provide suitable source cells in time to construct tissue engineered vascular grafts,the present study was designed to investigate the isolating and cryopreserving techniques of human umbilical vein endothelial cells in vitro. Methods Forty two human umbilical cords were used in this study.They were isolated by means of filling digestive enzyme solution into the lumen of umbilical veins.They were then cultured in M199 with 20% fetal bovine serum followed by immunofluorescence staining and scanning electron microscope examination.Then endothelial cells were suspended in cryopreserviong solution which contains 10% DMSO and 10% fetal bovine serum in M199 and were cryopreserved in liquid nitrogen.The proliferation of post-thawed cells and non-frozen cells were evaluated by measuring the metabloic activity of tetrazolium compound.The endothelial cell growth characteristics were determined by daily observation using phase contrast microscope.Post-thawed endothelial cells viability was determined by trypan blue staining test.Flow cytometry was used applied in this study to determin the apoptosis rate of post-thawed cells. Results Extremely high purified endothelial cells could be isolated by infusing digestive solutions to the lumen of human umbilical veins.Compared with non-frozen endothelial cells,postthawed endothelial cells showed 95% of vitality.No difference was found in growth curves of postthawed endothelial cells and non-frozen ones.The apoptosis rate of post-thawed endothelial cells was higher than that of non-frozen ones;however,there was no statistical difference. Conclusions Highly pure endothelial cells can be isolated by infusing digestive solutions to the lumen of human umbilical veins.Post-thawed endothelial cells are proved to have high vitality and growth potential in vitro.They may be an alterative choice of source cells for the construction of tissue engineered vascular graft." @default.
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- W2376972541 date "2004-01-01" @default.
- W2376972541 modified "2023-09-28" @default.
- W2376972541 title "Practice of Human Umbilical Endothelial Cell Isolation and Cryopreserving Technique" @default.
- W2376972541 hasPublicationYear "2004" @default.
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