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- W2377004865 abstract "To construct a recombinant plasmid contain CP15gene of Cryptosporidium parvum(C.parvum) and obtain recombinant protein.The surface antigen CP15gene fragment of C.parvum was amplified from plasmid pMD-CP15by PCR,and subcloned into pGEX 4T-3.The fusion express recombinant vector pGEX-CP15was constructed in E.coli BL21.The recombinant protein was induced by 1mmol / L IPTG and identified by SDS-PAGE and Western blotting.The CP15gene fragment was amplified correctly as the size of gene was about 390bp,and the recombinant plasmid pGEX-CP15was constructed.The protein band with a molecular weight of 42ku was detected on SDS-PAGE,which totally same with the theoretical size.The expressed protein was identified by Western blotting performed with GST serum.The fusion protein of CP15was highly expressed in E.coli." @default.
- W2377004865 created "2016-06-24" @default.
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- W2377004865 date "2012-01-01" @default.
- W2377004865 modified "2023-09-25" @default.
- W2377004865 title "Prokaryotic Expression and Identification of Cryptosporidium parvum Surface Antigen CP15 Gene in Escherichia coli BL21" @default.
- W2377004865 hasPublicationYear "2012" @default.
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