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- W2377008658 abstract "Objective To develop an enzyme immunoassay for measurement of the circulating levels of human adiponectin(hAPN) and evaluate its clinical significance.Methods Mouse anti-hAPN polyclonal antibodies(PcAb) with high titre were prepared by immunizing BALB/C mouse with recombinant hAPN.The hAPN protein was also absorbed to the microtitre strip wells as solid antigen,and competed with the liquid antigen in unknown sample to bind to the limited PcAb.A simple competitive ELA method was developed.Results The detectable low limit of the assay was 10 ug/L,and the working range was 10ug/L~800ug/L.Both of the inter-assay and intra-assay coefficient variation(CV) were less than 10%.Mean serum APN levels detected by us in-house EIA in health male subjects(11.7±7.4mg/L) were lower than the levels in female(16.2±6.8mg/L).Furthermore,as compared with health subjects of the same sex,APN levels in type 2 diabetes mellitus(4.3±2.0 mg/L in male,5.9±2.5mg/L in female) were much lower(P0.01).The results agreed well between our EIA and the commercially available Phoenix Pharmaceuticals ELISA(r =0.96,n=114).Conclusion With high quality the EIA assay provides us a new tool to study the clinical significance for measuring adiponectin concentraions in human serum." @default.
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- W2377008658 date "2007-01-01" @default.
- W2377008658 modified "2023-09-23" @default.
- W2377008658 title "Establishment and preliminary application of a competitive enzyme immunoassay for human adiponectin in serum" @default.
- W2377008658 hasPublicationYear "2007" @default.
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