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- W2377022174 abstract "The 4.2 kb cryptic plasmid was isolated from the standard reference strain of N.gonorrhoeae and purified by CsCl-ultracentrifugation.The 4.2 kb plasmid DNA was cloned into pUC 18 DNA Hind Ⅲ-site and then transformed into the E.coli JM103.A specific fragment of the cryptic plasmid was amplified by polymerase chain reaction to detect gonococcus.The cloned cryptic plasmid DNA from N.gonorrhoeae could be used for DNA probe as well the PCR positive controls.21 isolates of N.gonorrhoeae were positive by PCR, while the other 22 isolates of non-gonococcus were negative,It demonstrated that the PCR assay has a exquisite specificity.The samples collected from 69 high-risk patients were detected by three methods,microscope smear. cultured and PCR.The positve rate in PCR was 60.8%,much higher than that of microscope smear(26.08%) and cultured(18.84%).We examined 706 clinical specimens by PCR.the positive rate was 28.6%(202/706).Results show that the PCR is a simple,rapid,sensitive and specific method and is suited for routine assay." @default.
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- W2377022174 date "1995-01-01" @default.
- W2377022174 modified "2023-09-25" @default.
- W2377022174 title "CLONING OF THE CRYPTIC PLASMID FROM NEISSERIA GONORRHOEAE AND THE CLINICAL APPLICATION WITH PCR" @default.
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