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- W2377027610 abstract "Objective To compare the extraction effects of touch DNA by Chelex-100,DNA IQ and EQ magnetic beads.Methods The standard DNA diluted to 10ng,100ng was treated respectively by Chelex-100,DNA IQ system and EQ magnetic beads.DNA was extracted from 30 cigarette butts and 30 toothbrushes respectively by the three methods.The quality and quantity of DNA were detected between PCR quantification and STR analysis.Results There wasn't any loss of DNA with the Chelex method,but with DNA IQ method and the EQ magnetic beads method,some DNA was lost.For cigarette butts and toothbrushes samples,yield of touch DNA and IPC CT extracted by Chelex method are higher than that extracted by DNA IQ method and the EQ magnetic beads method,but they aren't significantly different by DNA IQ method and EQ magnetic beads method.Conclusion The Chelex method is very suitable for extracting DNA from the less pollution and low impurity samples,and the DNA IQ system and the EQ magnetic beads method are more suitable for minute and polluted samples." @default.
- W2377027610 created "2016-06-24" @default.
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- W2377027610 date "2012-01-01" @default.
- W2377027610 modified "2023-09-23" @default.
- W2377027610 title "The comparison of the extraction effects of touch DNA by the Chelex-100 and magnetic beads" @default.
- W2377027610 hasPublicationYear "2012" @default.
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