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- W2377046802 abstract "Objective To investigate the effect of total saponins of panax ginseng on expression of STAT5 in K562 cells.Methods Spectrophotometry and Wright′s staining were used to determine if TSPG induce K562 cells to differentiate into erythroid lineage cells.The expression of STAT5 protein in K562 cells was investigated by immunocytochemistry;STAT5 protein in cytosol fraction and cellular nucleus protein extracts of K562 cells were determinated by Elisa and Western blotting analysis.Results The level of hemoglobin in K562 cell induced by 200μg/ml TSPG is increased in time-dependent manner,AG490(40μmol/L) partly inhibited the rise of hemoglobin level in K562 cells.The cytoplasma and nucleus of K562 cell in control group is weakly stained for STAT5;after stimulated by 200μg/ml TSPG for 12h,both cytoplasma and nucleus of K562 cell is darkly stained(P0.05).Compared with control group,the amount of STAT5 in cytoplasma and nucleus of K562 cell induced by 200μg/ml TSPG is increased in time-dependent manner.Conclusion TSPG can enhance the expression of STAT5 in K562 cell,which may be one of mechanisms by which TSPG induces K562 cells to differentiate into erythroid lineage cells." @default.
- W2377046802 created "2016-06-24" @default.
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- W2377046802 date "2009-01-01" @default.
- W2377046802 modified "2023-09-26" @default.
- W2377046802 title "Effect of total saponins of panax ginseng on expression of STAT5 in K562 cells" @default.
- W2377046802 hasPublicationYear "2009" @default.
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