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- W2377048160 abstract "Objective To observe the effect of demethylation of DNA CpG island on the expression of runt-related transcription factor 3 gene(RUNX3) and the growth of T24 bladder cancer cell line,RUNX3 has the potential to become a new target for treatment of bladder cancer.Methods The status of methylation of RUNX3 gene in T24 bladder cancer cell line that was treated with 5-Aza-2'-deoxycytidine(5-Aza-CdR) was analyzed using methylation specific polymerase chain reaction(MSP) and un-methylation specific polymerase chain reaction(UMP).After treated with 5-Aza-CdR(0.5,5.0,50.0 μmol/L),the expression of RUNX3 mRNA was examined by semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR).Cell proliferation was evaluated by MTT assay,the apoptosis of T24 cells was analyzed by flow cytometry.Results The 5'CpG island methylation in RUNX3 gene promotor was detected in T24 bladder cancer cell line.After treated with 5-Aza-CdR,the promotor region of RUNX3 gene exhibits demethylation state,and the level of RUNX3 mRNA expression was increased(0.51±0.06、0.60±0.04、0.69±0.08),and it was significantly correlated with the concentration of 5-Aza-CdR(F=102.21,P0.01).5-Aza-CdR obviously decreased the proliferation and increased the apoptosis in T24 cells compared with those in control group(4.16%±1.56%、25.82%±1.81%、41.77%±3.25%、66.59%±3.16%,P0.01).The inhibition effect of 5-Aza-CdR was dosedependant(F=316.47,P0.01).Conclusions The 5'CpG island methylation is probably responsible for RUNX3 expression silencing in T24 bladder cancer cell line.5-Aza-CdR may effectively cause demethylation and inhibit the growth of tumor cell by reactivating the gene transcription silenced by aberrant hypermethylation." @default.
- W2377048160 created "2016-06-24" @default.
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- W2377048160 date "2009-01-01" @default.
- W2377048160 modified "2023-09-23" @default.
- W2377048160 title "Effects of 5-Aza-2'-deoxycytidine on expression of RUNX3 and biological behaviors of T24 bladder cancer cell line" @default.
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