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- W2377055461 abstract "Objective To improve a culture project for three-color detection of Th subgroup in peripheral blood by flow cytometry.Methods Peripheral whole blood was co-cultured with RPMI1640,25μg/L of PMA,1mg/L of Ionomycin and 20μg/L of Monensin for 2 hours,then the percentages of CD4~+ cells,CD69~+cells,IFN-γ~+of CD3 cells and IL-4~+of CD3 cells were detected by flow cytometry respectively.The percentages of CD4~+cells before cultured,IFN-γ~+of CD3 cells and IL-4~+of CD3 cells after cultured for 4 hours were detected by flow cytometry.Results CD4~+ cells and CD4~-cells can be discriminated by CD4 molecular of cell surface in lymphocyte gate.Compared with before cultured,the percentage of CD4~+cells after cultured for 2 hours was no difference(P0.05).CD69 were expressed by more than 90 percent of T cells.The percentages of IFN-γ~+of CD3 cells and IL-4~+ of CD3 cells cultured for 2 hours or 4 hours were no difference(P0.05).Conclusion The culture project can meet the requisition of CD4,IFN-γ and IL-4 three-color method for detecting Th subgroup in peripheral blood by flow cytometry." @default.
- W2377055461 created "2016-06-24" @default.
- W2377055461 creator A5069958936 @default.
- W2377055461 date "2006-01-01" @default.
- W2377055461 modified "2023-09-25" @default.
- W2377055461 title "Study of Culture Project for Three-Color Detection of Th-Subgroup in Peripheral Blood by Flow Cytometry" @default.
- W2377055461 hasPublicationYear "2006" @default.
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