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- W2377069352 abstract "Objective The recombinant plasmid containing the cytolethal distending toxin (CDT) gene of campylobacter jejuni (CJ) was constructed to provide a foundation for future development of C. jejuni DNA vaccine.Methods Primers were designed by Primer 5. 0 software according to CJ-cdtA, cdtB, cdtC gene sequences provided from GenBank. Polymerase chain reaction (PCR) was used to amplify the cdtA, cdtB, cdtC gene . The fragment was subcloned into appropriate site of pcDNA3. 1 ( + ) eukaryotic expression vector by restriction enzyme digestion and linking reactions. The positive recombinant was identified by restriction endonuclease digestion , PCR amplification and sequencing. Results The 807bp, 798bp, 570bp-cdtA, cdtB, cdtC specific gene fragments were amplified. Restriction enzyme analysis and sequencing showed that the eukaryotic expression recombinant pcDNA3. 1 ( + )-cdtA, cdtB, cdtC were successfully constructed. Conclusion The cdtA, cdtB, cdtC gene fragments are successfully amplified , and the eukaryotic expression recombinant pcDNA3. 1 ( + )-cdtA, cdtB, cdtC are successfully constructed ." @default.
- W2377069352 created "2016-06-24" @default.
- W2377069352 creator A5080756424 @default.
- W2377069352 date "2010-01-01" @default.
- W2377069352 modified "2023-09-23" @default.
- W2377069352 title "Construction and expression of eukaryotic expression recombinant vector of campylobacter jejuni cdtA、cdtB、cdtC gene" @default.
- W2377069352 hasPublicationYear "2010" @default.
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