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- W2377071139 abstract "A real-time polymerase chain reaction(PCR)assay was designed and evaluated for rapid detection and quantification of the toxic algae Heterocapsa circularisquama.A pair of specific primers was designed from the sequence of ITS1 region,of which the PCR specificity was examined compared with the other nine dinoflagellates.PCR amplifications were detected only from samples which contained H.circularisquama cells,and specific signals were not detected from any other microalgae.Based on crushed algae by ultrasound for the standard,the standard curve we got has high linearity,and correlation coefficient R2 was 0.989.Moreover,this assay can detect 0.5 cell within 30 cycles,showing its high sensitivity.DNAs from H.circularisquama that had been cultured in different conditions,were assayed in the run.Using the developed standard curves,H.circularisquama could be quantified in agreement with the quantification by optical microscopy." @default.
- W2377071139 created "2016-06-24" @default.
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- W2377071139 date "2010-01-01" @default.
- W2377071139 modified "2023-09-26" @default.
- W2377071139 title "Development of a real-time PCR assay for rapid detection and quantification of Heterocapsa circularisquama" @default.
- W2377071139 hasPublicationYear "2010" @default.
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