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- W2377087672 abstract "Bovine skeletal muscleα-actin mutation promoters were constructed by PCR site-specific mutagenesis to determine the functions of Sp1/KLFs,ZF5 F,Pax3binding sites located at-361--462bp;the reformed promoters containing introns were constructed by gene recombination technology.The dual luciferase expression vector were transfected to skeletal muscle satellite cells and bovine fetal fibroblast cells for activity detection.The results showed that,Sp1/KLFs could decrease promoter activity,ZF5 Fand Pax3could increase promoter activity.Introns located at the upstream of promoter increased the promoter activity,located at the downstream of promoter decreased the promoter activity.Cis-regulatory elements and introns had muscle-specificity.Analysis by PCR site-specific mutagenesis showed that cis-acting element Pax3 possessed as transcriptional repressor,Sp1/KLFs and ZF5 Fpossessed as transcriptional activators.It is found that the infulence of intron on bovine skeletalα-actin promoter activity is related to its location,which has laid a foundation for construction of bovine skeletalα-actin efficient promoter." @default.
- W2377087672 created "2016-06-24" @default.
- W2377087672 creator A5069639009 @default.
- W2377087672 date "2015-01-01" @default.
- W2377087672 modified "2023-09-26" @default.
- W2377087672 title "Functional analysis in the upstream cis-regulatory elements and introns of bovine skeletal α-actin gene" @default.
- W2377087672 hasPublicationYear "2015" @default.
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