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- W2377091738 abstract "Objective To observe the influences of the medical serum of Shumai Capsules and its different modified formulas on the proliferation of vascular smooth muscle cell(VSMC) induced by angiotensin II(Ang II) and cell cycle.Methods VSMC was cultured by using tissue explant method and Ang II(10-7 mol/L) was taken as stimulation factor.The medical serum were divided into Shumai Capsules group,modified formula group 1(with effect of activating blood circulation and resolving blood stasis) and modified group 2(with effect of replenishing spleen and kidney).The cell proliferation was detected by MTT test and cell cycle was examined by flow cytometer.Results The OD values in Shumai Capsules group and modified formula group 1 were lower than those in Ang II group and modified formula group 2 with a statistical difference.The outcomes of the flow cytometer showed that in G_O/G_1 phase the proportion of modified formula group 2 was lower than that of the blank group or Ang II group with a statistical difference.In S phase the proportion of the blank group,Shumai Capsules group or modified formula group 1 was lower than that of Ang II group or modified formula group 2 with a statistical difference.The proportion of Shumai Capsules group in S phase was higher than that of the blank group and lower than that of modified formula group 1.In G_2/M phase the proportion of modified formula group 1 and 2 was higher than that in blank group or Ang II group with a statistical difference.Conclusion Shumai Capsules can inhibit cell proliferation through stopping the translation of cell cycle from G_0/G_1 phase to S phase.The effect of Shumai Capsules was higher than that of modified formula with effect of activating blood circulation and resolving blood stasis.In modified formula group 2 the inhibition of cell proliferation was not observed but the improvement of cell proliferation was." @default.
- W2377091738 created "2016-06-24" @default.
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- W2377091738 date "2009-01-01" @default.
- W2377091738 modified "2023-09-27" @default.
- W2377091738 title "Influence of Shumai Capsules on proliferation of VSMC induced by Ang II and cell cycle" @default.
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