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- W2377096129 abstract "Objective:To investigate the mechanism of Mller cell adhesion to vitreous gel.Methods:Porcine Mller cells were extracted and cultured,identified with immuno-recognition with glial fibrillary acidic protein(GFAP),vimentin and glutamine syn-thetase(GS) antibody.Cells were collected and incubated with / without integrinα2β1 and CD44 antibody before application to fresh porcine vitreous for 2 hours,and then washed with PBS for 3 times.Remaining cells on the vitreous were counted.Results: ① Cells collected were 97.7% positive for GFAP,94.6% positive for vimentin,86.0% positive for GS,respectively.Cells adhered to vitreous after 2 hours of culture,formed net-like structure and enter vitreous gel after 72 hours;② Pre-incubation with integrinα2β1 antibody significantly decreased the remaining cell,while no such significance was observed in the cells incubated with CD44 antibody.Con-clusion:Mller cell can adhere to vitreous and proliferate,in which the interaction with type Ⅱ collagen is an important mechanism." @default.
- W2377096129 created "2016-06-24" @default.
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- W2377096129 date "2011-01-01" @default.
- W2377096129 modified "2023-09-23" @default.
- W2377096129 title "Interaction with collagen fiber is the main mechanism of Mller cell adhesion to vitreous gel" @default.
- W2377096129 hasPublicationYear "2011" @default.
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