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- W2377100143 abstract "AIM To observe the cellular viability and cell proliferative capacity of human aortic valves cryopreserved in liquid nitrogen at -196℃. METHODS Twenty valves, procured from 18 to 35 years old healthy adult hearts within 30 min after the determination of brain death, were divided into 10 groups. Group Ⅰ, the control group, is of fresh valves. Valves from Group Ⅱ to Group Ⅹ were those preserved in liquid nitrogen for 1, 3, 6, 9, 12, 15, 18, 21, 24 mo respectively. The fresh valves were examined immediately after procurement. All the cryopreserved valves were thawed in 37℃ saline pool. Each specimen was cut into pieces of 1 mm×1 mm×1 mm. Half of the tissues were reduced with 2 5 g·L -1 trypsin and dyed with trypan blue, then the number of viable and nonviable cells were counted. The remaining tissues were incubated in RPMI1640 medium containing 100 mL·L -1 fetal calf serum at 37℃ to observe the capacity of cell proliferation on the 7th and 28th day. RESULTS Fresh valves seemed to have the highest cell survival rate of all the groups (93.8%). In cryopreservation groups, there was a tendency that the longer the duration of preservation was, the lower the survival rate was. Valves from Group Ⅱ to Group Ⅹ showed a significant decrease in cell survival rate compared with those from Group Ⅰ(67.3% vs 93 8% P 0 05). Valves from Group Ⅷ, Ⅸ and Ⅹ showed a significant decrease in cell survival rate compared with those from Group Ⅰ to Group Ⅶ (52.4% vs 76.5% P 0.05). Cells in Group Ⅰ grew out of the tissue pieces during the first week and fully covered the bottom of culture container during the fourth week. In the middle of the second week, however, cells of Group Ⅱ to Group Ⅶ started to appear at the edge of the tissues and occupied the most part of the container bottom. In Group Ⅷ, Ⅸ and Ⅹ, cells were scarcely seen during the first week and the cell number was markedly decreased during the fourth week compared with that of the fresh valves. CONCLUSION Cryopreservation in liquid nitrogen has significant effects on the cellular viability and cell proliferative capacity of human aortic valve tissues. Valves that are preserved for 18 or more months have lower cellular survival rates and show a dramatic decrease in cellular capacity of proliferation. According to the results of our experiments, the durability of valves cryopreserved 18 or more months would decrease correspondingly." @default.
- W2377100143 created "2016-06-24" @default.
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- W2377100143 date "2001-01-01" @default.
- W2377100143 modified "2023-09-23" @default.
- W2377100143 title "Influence of cryopreservation on tissue viability of human aortic valve homografts" @default.
- W2377100143 hasPublicationYear "2001" @default.
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