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- W2377126502 abstract "OBJECTIVE: To explore the apoptosis mechanism of HL-60 cell induced by TSA.METHODS: The MTT method was used to test HL-60 cell growth.The cell cycle and apoptosis were tested by FCM.Semi-quantitative RT-PCR was used to detect the mRNA expressions of Bax,Caspase-9 and Caspase-3 in the cells treated by TSA.RESULTS: A decrease in cell growth was observed when the cells were treated by 0.1 μmol/L TSA,P0.01.The cells were significantly accumulated at the G0/G1 phase under the treatment of 0.05 μmol/L TSA(P0.05).However,the But cell apoptosis was only observed when the concentration of TSA was over 0.1 μmol/L(P0.01).The expressions of Bax,Caspase-9 and Caspase-3 were up regulated by the exposure of HL-60 cells to 0.1 μmol/L TSA for 12 hours,P0.01.CONCLUSION: The mechanism of HL-60 cell apoptosis induced by TSA is related to the up-regulation of Bax,Caspase-9 and Caspase-3." @default.
- W2377126502 created "2016-06-24" @default.
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- W2377126502 date "2009-01-01" @default.
- W2377126502 modified "2023-09-23" @default.
- W2377126502 title "Apoptosis mechanism of HL-60 cells induced by TSA" @default.
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