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- W2377145110 abstract "Response surface methodology was applied to optimize medium constituents for enzymatic production of(S)-α-ethyl-2-O-1-pyrrolidineacetic acid from racemic α-ethyl-2-O-1-pyrrolidineacetic acid ethyl ester by a novel Tsukamurella tyrosinosolvens E105.The influences of carbon and nitrogen resources were investigated.Then the Plackett-Burman design experiment was used to evaluate the effects of different components in the culture medium,yeast extract and initial pH were found to be the most important factors among seven tested variables affecting enzyme activity.Central composite design and response surface analysis were subsequently employed for further optimization,and the optimal concentration of yeast extract was 12.1 g/L,with the optimal initial pH of 7.06.Under the optimum conditions,the maximum enzyme activity of 1024.6 U/mL in the experiment was obtained,corresponding to an increase of 67% in comparison with the original medium components.Medium optimization for Tsukamurella tyrosinosolvens E105 could increase obviously its hydrolyase-producing capability of enzymatic preparation of(S)-α-ethyl-2-O-1-pyrrolidineacetic acid." @default.
- W2377145110 created "2016-06-24" @default.
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- W2377145110 date "2011-01-01" @default.
- W2377145110 modified "2023-09-23" @default.
- W2377145110 title "Medium optimization for α-ethyl-2-O-1-pyrrolidineacetic acid ethyl ester hydrolase-producing strain using response surface methodology" @default.
- W2377145110 hasPublicationYear "2011" @default.
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