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- W2377196384 abstract "Heat shock protein 70(HSP) is widely distributed in organisms and plays important roles in protein transport,heat resistance and cell protection.In this study,an HSP70 amino acid sequence of Drosophila melanogaster was used to do Blastp search in the silkworm genome.Several putative HSP70 genes were found.We chose one of the gene(accession number: BGIBMGA007950) for electronic cloning.The putative coding sequence of BGIBMGA002381 was used to do Blastn search in the silkworm expressed sequence tags(ESTs) database.A total of 20 ESTs were obtained and assembled by SeqMan.The cDNA sequence of BGIBMGA002381 was 2057 bp in length.We predicted that the coding sequence of BGIBMGA002381 was 1950 bp in length,coding 649 amino acids.Its molecular weight and isoelectric point were 71.18 kD and 5.12,respectively.The results suggested that BGIBMGA002381 might be an active gene in the silkworm genome." @default.
- W2377196384 created "2016-06-24" @default.
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- W2377196384 date "2011-01-01" @default.
- W2377196384 modified "2023-09-24" @default.
- W2377196384 title "Electronic Cloning and Sequence Analysis of HSP70 Gene in the Silkworm" @default.
- W2377196384 hasPublicationYear "2011" @default.
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