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- W2377220475 abstract "Tetraploids in Jinjiang oyster, Crassostrea rivularis, were induced by inhibiting the first polar body (PB1) with cytochalasin B (CB). Increasing temperature from 25℃ to 28℃ led to an increase in the synchrony of zygote development. The optimal dosage of CB for tetraploid induction was 0.6mg/L at 28℃. The following timing of CB treatment was effective for tetraploid production: starting when the first signs of PB1 extrusion were observed and ending as 50% of fertilized eggs in control group expulsed PB1. On average, the yield of D-stage larvae in treatment groups was 11.0%, which was significantly lower than that of controls (72.4%) (P0.01). Most mortality occurred to the induced larvae during the first 8—10 days post fertilization (PF). Compared to controls, average larval survival of treatment groups was significantly lowered during the period of 4—12 days PF in all five replicates (P0.05). Tetraploids were obtained in both embryos and D-stage larvae from five replicates. The proportion of tetraploids varied from 37% to 48%, averaging 41.8% in embryos, as confirmed by chromosome counting. Diploids, triploids and aneuploids were observed in CB-treatment groups. Flow cytometric analysis of D-stage larvae of treatment groups revealed ploidy compositions similar to that of embryos. The average percentage of diploids, triploids, tetraploids and aneuploids was 21.3%, 18.2%, 37.9% and 22.5%, respectively, in D-stage larvae. The percentage of tetraploids decreased over the time of larval development. Tetraploids were undetectable at Day 12 PF. Two of five replicates of CB-treatment groups survived beyond settlement and metamorphosis. The average cumulative survival from fertilized eggs to spat was 0.3% for CB-treated groups. Tetraploids were not found in spat, indicating the weak survival of tetraploid larvae. Overall, tetraploids can be effectively induced by blocking PB1 with CB treatment. However, how to enhance the survival of tetraploid larvae remains a challenge for future research." @default.
- W2377220475 created "2016-06-24" @default.
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- W2377220475 date "2005-01-01" @default.
- W2377220475 modified "2023-09-25" @default.
- W2377220475 title "TETRAPLOIDS INDUCED BY INHIBITING POLAR BODY I WITH CYTOCHALASIN B IN JINJIANG OYSTER, CRASSOSTREA RIVULARIS" @default.
- W2377220475 hasPublicationYear "2005" @default.
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