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- W2377221790 abstract "A CAD gene was cloned from the young stem of Eucalyptus urophylla clone GLU4 and named EuCAD by using specific primers based on the highly conserved sequences of plant CAD. Sequence analysis indicated that its gDNA and cDNA sequence were 2 200 bp and 1 102 bp respectively,which open reading frame encoding 356 amino acid residues. EuCAD encoding sequence was analyzed,and the result showed it has an entire CAD1 domain, a NADP binding site and two Zn binding sites. The results of homology analysis showed that EuCAD nucleotide sequence had more than 97 % sequence homology with that of other 5 Eucalyptus,and EuCAD encoding protein had more than 79 % homology with that of Prunus persica and others. The physicochemical property and structure of EuCAD encoding protein were analyzed using bioinformatics tools. The result showed it was an acidic protein without signal peptide or transmembrane domain,and its secondary structure has typical α-helix and β-sheet. Tertiary structure modeling showed it was most similar to AtCAD4 in Arabidopsis thaliana. Phylogenetic analysis showed that it has close relative relation with Eucalyptus and Populus,but distant relation with Taxodiaceae." @default.
- W2377221790 created "2016-06-24" @default.
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- W2377221790 date "2014-01-01" @default.
- W2377221790 modified "2023-09-25" @default.
- W2377221790 title "Cloning and Prokaryotic Expression of Cinnamyl Alcohol Dehydrogenase of Eucalyptus urophylla Clone GLU4" @default.
- W2377221790 hasPublicationYear "2014" @default.
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