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- W2377246431 abstract "Dihydroflavonol 4-reductase(DFR) catalyzes the last common step in the anthocyanin biosynthesis pathway.By using PCR,substrate binding region of DFR from ornamental sunflower(Helianthus annuus) was knocked out,and-134 conserved amino acid residue asparagine was mutated into aspartate or leucine.KODFR,mutated N134D and N134L were successfully amplified.Plant expression vector pCAM-KODFR,pCAM-N134D and pCAM-N134L were also successfully constructed,which will be used for gene transformation." @default.
- W2377246431 created "2016-06-24" @default.
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- W2377246431 date "2008-01-01" @default.
- W2377246431 modified "2023-09-28" @default.
- W2377246431 title "PCR-mediated modification of DFR gene from ornamental sunflower and construction of the gene′s plant expression vector" @default.
- W2377246431 hasPublicationYear "2008" @default.
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