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- W2377278870 abstract "【Objective】The study aimed to screen fibroblasts with deficiency ofα1,3galactosyl transferase gene(GGTA1).【Method】After being transfected with knockout vectors pBS-GGTA1-SKO and pBS-GGTA1-DKO,GGTA1+/-fetal fibroblasts of Wuzhishan miniature pigs(WZSP)were treated with Phytolectin GSI-B4.Magnetic activated cell sorting(MACS),combination of MACS with fluorescence-activated cell sorting(FACS),and combination of Clostridium difficile Toxin A(TcdA)with FACS methods were used to screen the cells.Single-cell clones screened by MACS were identified by PCR and FACS. Colonies obtained by combination of MACS with FACS and combination of TcdA with FACS were identified using PCR.【Result】11colonies were screened by MACS and 2of them were identified as GGTA1-/-cells with a positive rate of 18.2%.3cell collections were identified by combination of MACS with FACS with a positive rate of 0.Combination of TcdA with FACS obtained 3cell collections with a positive rate of 100%.【Conclusion】MACS and combination of TcdA with FACS could efficiently screen WZSP fibroblasts with deficiency of GGTA1gene.This study would improve further study on genetic modification of pigs for xenotransplantation." @default.
- W2377278870 created "2016-06-24" @default.
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- W2377278870 date "2014-01-01" @default.
- W2377278870 modified "2023-09-23" @default.
- W2377278870 title "Efficient screen of porcine fibroblasts with GGTA1 gene deficiency (GGTA1~(-/-)) using αGal epitopes difference" @default.
- W2377278870 hasPublicationYear "2014" @default.
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