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- W2377295027 abstract "Objective: The high performance liquid chromatography(HPLC) method of precolumn derivatization with 1-phenyl-3-methyl-5-pyranozolone(PMP) was developed to efficiently separate several monosaccharides.And the monosaccharide composition and content of polysaccharides in the medicine pair of Dioscorea opposita and Rehmannia glutinosa and their single herb medicine were determined and compared.Method: A Hypersil ODS2 column with the mobile phase of 0.05 mol.L-1 phosphate buffer(pH 6.7) acetonitrile(83∶ 17) at the detection wavelength of 254 nm was used.The flow rate was 1 mL.min-1,the column temperature was kept at 30 ℃.Result: All three polysaccharides contained mannose,rhamnose,glucuronide acid,glucose,galactose and arabinose.However,fucose was not detected.At the same time,it was found that the content of mannose,rhamnose,glucuronide acid,glucose,galactose and arabinose in their medicine pair was between in the single Dioscorea opposita polysaccharides and Rehmannia glutinosa polysaccharides.Conclusion: The developed method is simple,rapid and accurate.Therefore,it is an efficient method to analyze the monosaccharide composition in different kinds of polysaccharides in drug and food." @default.
- W2377295027 created "2016-06-24" @default.
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- W2377295027 date "2013-01-01" @default.
- W2377295027 modified "2023-09-25" @default.
- W2377295027 title "Comparision of the Composition and Content of Monosaccharide in Dioscorea opposita,Rehmannia glutinosa and the Medicine Pair" @default.
- W2377295027 hasPublicationYear "2013" @default.
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